MYC-tag Monoclonal antibody
MYC-tag Monoclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Recombinant protein and More (3)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
2D11A8
Cat no : 66004-1-Ig
Synonyms
"MYC-tag Antibodies" Comparison
View side-by-side comparison of MYC-tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 28 publications below |
| IF | See 3 publications below |
| IP | See 7 publications below |
| CoIP | See 4 publications below |
Product Information
66004-1-Ig targets MYC-tag in WB, IP, IF, CoIP, ELISA applications and shows reactivity with Recombinant protein samples.
| Tested Reactivity | Recombinant protein |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | MYC-tag |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2881489 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IF protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| IP protocol for MYC-tag antibody 66004-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell PTPS Facilitates Compartmentalized LTBP1 S-Nitrosylation and Promotes Tumor Growth under Hypoxia. | ||
Circ Res Yes-Associated Protein Promotes Angiogenesis Via Signal Transducer and Activator of Transcription 3 in Endothelial Cells. | ||
Nat Commun The ubiquitin ligase UBR5 suppresses proteostasis collapse in pluripotent stem cells from Huntington's disease patients. | ||
Cell Res ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. |
