MYC tag Monoclonal antibody, PBS Only

MYC tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A2

Cat no : 60003-2-PBS

Synonyms

myc, EQKLISEEDL

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Validation Data Gallery

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  WB analysis of recombinant proein using 60003-2-Ig (same clone as 60003-2-PBS)

  Western blot of Myc-tagged fusion protein with anti-Myc-tag (60003-2-Ig) at various dilutions. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

  IP result of anti-MYC tag (IP:60003-2-Ig, 4ug; Detection:60003-2-Ig 1:10000) with Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 60003-2-Ig (same clone as 60003-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using MYC tag antibody (60003-2-Ig, Clone: 1A5A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 transfected with GFP-MYC using 60003-2-Ig (same clone as 60003-2-PBS)

  1x10^6 HEK-293 transfected with GFP-MYC were intracellularly stained with 0.25 ug MYC tag Monoclonal antibody (60003-2-Ig, Clone:1A5A2) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60003-2-PBS in a different storage buffer formulation.

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Product Information

60003-2-PBS targets MYC tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: MYC tag
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The c-Myc tag corresponds to amino acid residues(EQKLISEEDL) of the human c-Myc protein. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. Myc-Tag mouse mAb detects recombinant proteins containing the Myc tag. The antibody recognizes the Myc-tag EQKLISEEDL fused to either the amino- or carboxy- terminus of targeted proteins.