CoraLite® Plus 488-conjugated MDC1 Recombinant antibody

MDC1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240469D5

Cat no : CL488-83496

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

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Validation Data Gallery

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  IF Staining of HeLa using CL488-83496

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MDC1 antibody (CL488-83496, Clone: 240469D5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-83496 is MDC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDC1 fusion protein Ag20135
• Full Name: mediator of DNA damage checkpoint 1
• Calculated Molecular Weight: 2089 aa, 227 kDa
• Observed Molecular Weight: 260-320 kDa
• GenBank Accession Number: BC152556
• Gene Symbol: MDC1
• Gene ID (NCBI): 9656
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.