MDC1 Recombinant antibody

MDC1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240469D5

Cat no : 83496-1-RR

Synonyms

NFBD1, NFBD 1, MDC 1, 240469D5

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Validation Data Gallery

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  WB analysis of human testis using 83496-1-RR

  human testis tissue were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83496-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83496-1-RR (MDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 83496-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MDC1 antibody (83496-1-RR, Clone: 240469D5 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83496-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83496-1-RR against Human MDC1 were performed. The affinity constant is 0.346 nM.

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Tested Applications

• Positive WB detected in: human testis tissue, HeLa cells, HEK-293 cells, MCF-7 cells, K-562 cells, PC-3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83496-1-RR is MDC1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDC1 fusion protein Ag20135
• Full Name: mediator of DNA damage checkpoint 1
• Calculated Molecular Weight: 2089 aa, 227 kDa
• Observed Molecular Weight: 260-320 kDa
• GenBank Accession Number: BC152556
• Gene Symbol: MDC1
• Gene ID (NCBI): 9656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDC1, also named as KIAA0170 or NFBD1, is a 2089 amino acid protein, which contains two BRCT domains and one FHA domain. MDC1 localizes in the nucleus and is highly expressed in testis. MDC1 is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. MDC1 may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked.