APC Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

2A3

Cat no : APC-65209

Synonyms

IgG2a, IgG2a Isotype Control, Rat IgG2a Isotype Control

---

Validation Data Gallery

• 

  FC experiment of BALB/c mouse splenocytes using APC-65209

  1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug APC Rat IgG2a Isotype Control (APC-65209, Clone: 2A3) (blue) or 0.5 ug APC Anti-Mouse CD4 (APC-65141, Clone: RM4-5) (red). Cells were not fixed.

---

Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: IgG2a Isotype Control
• RRID: AB_2883050
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.