FITC Rat IgG2a Isotype Control (2A3)
IgG2a Isotype Control Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
2A3
Cat no : FITC-65209
Synonyms
Validation Data Gallery
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Tested Applications
| Positive FC detected in | BALB/c mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 1 publications below |
Product Information
FITC-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.
| Tested Reactivity | |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | N/A |
| Full Name | IgG2a Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883823 |
| Conjugate | FITC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 494 nm / 520 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC IgG2a Isotype Control antibody FITC-65209 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation |
