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APC Anti-Mouse CD4 (RM4-5)

CD4 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

RM4-5

Cat no : APC-65141

Synonyms

Cd4, CD4 antigen, L3T4, Ly 4

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using APC Anti-Mouse CD4 (RM4-5) (APC-65141)

    FC experiment of mouse splenocytes using APC-65141

    1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC Anti-Mouse CD4 (APC-65141, Clone: RM4-5). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using APC Anti-Mouse CD4 (RM4-5) (APC-65141)

    FC experiment of mouse splenocytes using APC-65141

    1X10^6 C57BL/6 mouse splenocytes were surface stained with FITC-Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.13 ug APC-Rat IgG2a isotype control antibody. Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using APC Anti-Mouse CD4 (RM4-5) (APC-65141)

    FC experiment of mouse splenocytes using APC-65141

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.20 ug APC-anti-mouse CD4 (APC-65141, clone RM4-5) (red) or 0.20 ug APC-rat IgG2a isotype control (blue). Cells were not fixed. .

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65141 targets CD4 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen BALB/c mouse thymocytes
Full Name CD4 antigen
GenBank Accession NumberBC039137
Gene Symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883022
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
FC protocol for APC CD4 antibody APC-65141Download protocol
Standard Protocols
Click here to view our Standard Protocols