FC experiment of BALB/c mouse splenocytes using CL594-65209
1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Rat IgG2a Isotype Control (CL594-65209, Clone: 2A3) (red), or 0.5 ug CoraLite®594 Anti-Mouse CD16/32 (93) (CL594-65057, Clone: 93) (green) or unstained (blue). Cells were not fixed.
1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Rat IgG2a Isotype Control (CL594-65209, Clone: 2A3) (red), or 0.5 ug CoraLite®594 Anti-Mouse CD16/32 (93) (CL594-65057, Clone: 93) (green) or unstained (blue). Cells were not fixed.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
CL594-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
Background Information
The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
Product Specific Protocols
FC protocol for CL594 IgG2a Isotype Control antibody CL594-65209
FC experiment of BALB/c mouse splenocytes using CL594-65209
1X10^6 BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite®594 Anti-Rat IgG2a Isotype Control (CL594-65209, Clone: 2A3) (red), or 0.5 ug CoraLite®594 Anti-Mouse CD16/32 (93) (CL594-65057, Clone: 93) (green) or unstained (blue). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IgG2a Isotype Control Monoclonal antibody
Catalog Number
CL594-65209
Citations
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Dilutions
Applications
FC
Reactivity
-
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
