FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

HLA-DR Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

L243

Cat no : FITC-65560

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

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Validation Data Gallery

• 

  FC experiment of human PBMCs using FITC-65560

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD14 and 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-65560

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (FITC-65560, Clone: L243) (red), or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.