Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using Biotin-65560

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (Biotin-65560, Clone: L243) and CoraLite®488-conjugated streptavidin, or unstained. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were not fixed.

• 

  FC experiment of human PBMCs using Biotin-65560

  1x10^6 human PBMCs were surface stained with 5 ul Biotin Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (Biotin-65560, Clone: L243) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed.

HLA-DR Uni-rAb^TM Recombinant Antibody for FC

Cat No. Biotin-65560

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• RRID: AB_3672372
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).