CoraLite® Plus 750 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody

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  FC experiment of human PBMCs using CL750-65560

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD14 and 5 ul CoraLite® Plus 750 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL750-65560, Clone: L243), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-65560

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody (CL750-65560, Clone: L243) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

HLA-DR Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-65560

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

HLA-DRA, L243, HLA-DRA1, HLA DRB, HLA DRA1

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-65560 targets HLA-DR in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen: Human lymphoblastoid B-cell line RPMI 8866
• Full Name: major histocompatibility complex, class II, DR alpha
• Calculated Molecular Weight: 254 aa, 29 kDa
• GenBank Accession Number: BC032350
• Gene Symbol: HLA-DRA
• Gene ID (NCBI): 3122
• ENSEMBL Gene ID: ENSG00000204287
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01903
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903).