CoraLite® Plus 647 Anti-Human HLA-DR (L243) Mouse IgG2a Recombinant Antibody
HLA-DR Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
L243
Cat no : CL647-65560
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | human PBMCs |
Recommended dilution
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-65560 targets HLA-DR in FC applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG2a |
Class | Recombinant |
Type | Antibody |
Immunogen | Human lymphoblastoid B-cell line RPMI 8866 |
Full Name | major histocompatibility complex, class II, DR alpha |
Calculated Molecular Weight | 254 aa, 29 kDa |
GenBank Accession Number | BC032350 |
Gene Symbol | HLA-DRA |
Gene ID (NCBI) | 3122 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 647 HLA-DR antibody CL647-65560 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |