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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11308

Synonyms

Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, GOLGA2/GM130, Golgi Marker, Golgin 95, Golgin subfamily A member 2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated GOLGA2/GM130 Polyclo (CL488-11308)

    IF Staining of HeLa using CL488-11308

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200, CoraLite®594 Coilin antibody (CL594-10967, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated GOLGA2/GM130 Polyclo (CL488-11308)

    IF Staining of HepG2 using CL488-11308

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 GOLGA2/GM130 antibody (CL488-11308) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-11308 targets GOLGA2/GM130 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight 111 kDa
GenBank Accession NumberBC014188
Gene Symbol GM130
Gene ID (NCBI) 2801
RRIDAB_2919024
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GOLGA2/GM130 antibody CL488-11308Download protocol
Standard Protocols
Click here to view our Standard Protocols