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CoraLite®594-conjugated Coilin Polyclonal antibody

Coilin Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10967

Synonyms

COIL, p80-coilin, p80 coilin, CLN80

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Coilin Polyclonal antibody (CL594-10967)

    IF Staining of HeLa using CL594-10967

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Coilin antibody (CL594-10967) at dilution of 1:200, (CL488-Phalloidin, green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated Coilin Polyclonal antibody (CL594-10967)

    IF Staining of HeLa using CL594-10967

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 Coilin antibody (CL594-10967) at dilution of 1:200, CoraLite®488 GOLGA2/GM130 antibody (CL488-11308, green).

  • Flow cytometry (FC) experiment of HEK-293T cells using CoraLite®594-conjugated Coilin Polyclonal antibody (CL594-10967)

    FC experiment of HEK-293T using CL594-10967

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Coilin (CL594-10967) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-10967 targets Coilin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Coilin fusion protein Ag1416
Full Name coilin
Calculated Molecular Weight 576 aa, 63 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC010385
Gene Symbol COIL
Gene ID (NCBI) 8161
RRIDAB_2919756
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

The nuclear coiled body(CBs) and nuclear structures known as gems contain high concentrations of the survival motor neurons (SMN) protein complex. CBs and gems often colocalize, and communication between them is mediated by COIL. COIL is a component of the CBS which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBs disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin, and symmetrical dimethylation of the COIL RG box motif is a molecular switch that determines whether the SMN complex forms gems or becomes incorporated into CBs

Protocols

Product Specific Protocols
IF protocol for CL594 Coilin antibody CL594-10967Download protocol
FC protocol for CL594 Coilin antibody CL594-10967Download protocol
Standard Protocols
Click here to view our Standard Protocols