GOLGA2/GM130 Polyclonal antibody

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  WB analysis of HeLa using 11308-1-AP

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.

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  WB analysis of HEK-293 using 11308-1-AP

  WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

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  WB analysis using 11308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11308-1-AP

  HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 11308-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 11308-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 11308-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 11308-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  IF Staining of MDCK using 11308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11308-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GOLGA2/GM130 Polyclonal antibody (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 11308-1-AP

Publications(138)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, canine and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GM130, GOLGA2, Golgin 95, GM130;GOLGA2, GM130 autoantigen

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human spleen tissue, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MDCK cells, HEK-293 cells, HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 75 publications below
• IHC: See 3 publications below
• IF: See 66 publications below
• IP: See 1 publications below

Product Information

11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, canine samples.

• Tested Reactivity: human, canine
• Cited Reactivity: human, monkey, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOLGA2/GM130 fusion protein Ag1848
• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• RRID: AB_2115327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08379
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publications

Species	Application	Title
	WB	Nature; Gasdermin D-mediated metabolic crosstalk promotes tissue repair; Authors - Zhexu Chi
human	IF	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
		Immunity; The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity; Authors - Tian Chen
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
human	IF	Adv Mater; Supramolecular Hydrogel with Ultra-Rapid Cell-Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration; Authors - Zhuo Li
	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-02-2023)

very good for WB.

• Applications: Western Blot

FH

Stephen (Verified Customer) (10-01-2019)

RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: RPE (human)