Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human spleen tissue, HeLa cells, MCF-7 cells, MDCK cells|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, HepG2 cells, MDCK cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:2000-1:8000|
|Sample-dependent, check data in validation data gallery|
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, Canine samples.
|Tested Reactivity||human, Canine|
|Cited Reactivity||human, Hamster, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GOLGA2/GM130 fusion protein Ag1848|
|Full Name||golgi autoantigen, golgin subfamily a, 2|
|Calculated molecular weight||111 kDa|
|Observed molecular weight||130 kDa|
|GenBank accession number||BC014188|
|Gene ID (NCBI)||2801|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Stephen (Verified Customer) (10-01-2019)
RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours GM130 antibody (green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA