CoraLite®594-conjugated GOLGA2/GM130 Polyclonal antibody

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  IF Staining of HepG2 using CL594-11308

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GOLGA2/GM130 antibody (CL594-11308) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-11308

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human GOLGA2/GM130 (CL594-11308) (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

GOLGA2/GM130 Polyclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL594-11308

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-11308 targets GOLGA2/GM130 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1848

  Product name: Recombinant human GM130;GOLGA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 361-678 aa of BC014188

  Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI

• Full Name: golgi autoantigen, golgin subfamily a, 2
• Calculated Molecular Weight: 111 kDa
• GenBank Accession Number: BC014188
• Gene Symbol: GM130
• Gene ID (NCBI): 2801
• RRID: AB_2919763
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08379
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein, one component of the detergent and salt-resistant Golgi matrix. It is a peripheral membrane protein highly bound to the Golgi membrane and localized mainly at the cytoplasmic face of the cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in regulating ER-to-Golgi transport and maintaining the Golgi structure. Emerging evidence suggests that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher-order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; EGFR core fucosylation, induced by hepatitis C virus, promotes TRIM40-mediated-RIG-I ubiquitination and suppresses interferon-I antiviral defenses; Authors - Qiu Pan