CoraLite®594-conjugated GOLGA2/GM130 Polyclonal antibody

GOLGA2/GM130 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-11308

Synonyms

GM130, GM130 autoantigen, GOLGA2, GOLGA2/GM130, Golgi Marker, Golgin 95, Golgin subfamily A member 2



Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11308-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-11308 targets GOLGA2/GM130 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
GenBank accession numberBC014188
Gene symbol GOLGA2
Gene ID (NCBI) 2801
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm / 614 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
IF protocol for CL594 GOLGA2/GM130 antibody CL594-11308Download protocol
Standard Protocols
Click here to view our Standard Protocols