CUL4B Recombinant antibody, PBS Only (Detector)

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  IF Staining of HeLa using 85001-4-RR (same clone as 85001-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85001-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85001-4-RR (same clone as 85001-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CUL4B Recombinant antibody (85001-4-RR, Clone:242626F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85001-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01736-2

  Cytometric bead array standard curve of MP01736-2, CUL4B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85001-3-PBS. Detection antibody: 85001-4-PBS. Standard: Ag3563. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 85001-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

CUL4B {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 85001-4-PBS

Clone No.242626F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

242626F11, cullin 4B, Cullin-4B, KIAA0695

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Product Information

85001-4-PBS targets CUL4B as part of a matched antibody pair:

MP01736-2: 85001-3-PBS capture and 85001-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CUL4B fusion protein Ag3563
• Full Name: cullin 4B
• Calculated Molecular Weight: 913 aa, 104 kDa
• GenBank Accession Number: BC036216
• Gene Symbol: CUL4B
• Gene ID (NCBI): 8450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13620
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).