CUL4B Recombinant antibody

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  IF Staining of HeLa using 85001-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 85001-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

CUL4B Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Cat No. 85001-4-RR

Clone No.242626F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

KIAA0695, Cullin-4B, cullin 4B, 242626F11

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85001-4-RR targets CUL4B in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CUL4B fusion protein Ag3563
• Full Name: cullin 4B
• Calculated Molecular Weight: 913 aa, 104 kDa
• GenBank Accession Number: BC036216
• Gene Symbol: CUL4B
• Gene ID (NCBI): 8450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13620
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).