CoraLite®594-conjugated CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C4B2

Cat no : CL594-66995

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66995

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 CTBP1 antibody (CL594-66995, Clone: 1C4B2 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66995

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human CTBP1 (CL594-66995, Clone:1C4B2) (red), or 0.2 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66995 targets CTBP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CTBP1 fusion protein Ag28567
• Full Name: C-terminal binding protein 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC011655
• Gene Symbol: CTBP1
• Gene ID (NCBI): 1487
• RRID: AB_2883636
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011