Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 66995-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HEK-293 using 66995-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 66995-1-Ig
A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 66995-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 66995-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 66995-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66995-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC experiment of HepG2 using 66995-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC
IF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
66995-1-Ig targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 66995-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of HEK-293 using 66995-1-Ig
HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 66995-1-Ig
A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 66995-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 66995-1-Ig
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 66995-1-Ig
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 66995-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).
IF Staining of HepG2 using 66995-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HepG2 using 66995-1-Ig
1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
Proteintech
CTBP1 Monoclonal antibody
Catalog Number
66995-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF/ICC : 1:2000-1:8000 FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
