CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C4B2

Cat no : 66995-1-Ig

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

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Validation Data Gallery

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  WB analysis using 66995-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66995-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 66995-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 66995-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 66995-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66995-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66995-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66995-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66995-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66995-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66995-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 66995-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HepG2 using 66995-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HEK-293 cells, A549 cells, HL-60 cells, HSC-T6 cells, HeLa cells, NCCIT cells, LNCaP cells, Jurkat cells, 4T1 cells, RAW 264.7 cells, NIH/3T3 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:2000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66995-1-Ig targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CTBP1 fusion protein Ag28567
• Full Name: C-terminal binding protein 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: BC011655
• Gene Symbol: CTBP1
• Gene ID (NCBI): 1487
• RRID: AB_2882312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

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PF00011