FITC Plus Anti-Human CD68 (KP1)

• 

  FC experiment of human PBMCs using FITC-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65202

  1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (right), or Mouse IgG1 Isotype Control (left). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

CD68 Monoclonal Antibody for FC (Intra)

Cat No. FITC-65202

Clone No.KP1

Publications(2)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC (Intra)

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

---

Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

FITC-65202 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_2924132
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P34810
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

Species	Application	Title
human	FC (Intra)	Front Bioeng Biotechnol; Evaluation of nanoparticle albumin-bound paclitaxel loaded macrophages for glioblastoma treatment based on a microfluidic chip; Authors - Zuorun Xie
	FC	Int Immunopharmacol; FOXF2 suppressed esophageal squamous cell carcinoma by reducing M2 TAMs via modulating RNF144A-FTO axis; Authors - Tianci Han