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FITC Plus Anti-Human CD68 (KP1)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC (Intra)

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

KP1

Cat no : FITC-65202

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (KP1) (FITC-65202)

    FC experiment of human PBMCs using FITC-65202

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (red), or Mouse IgG1 Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD68 (KP1) (FITC-65202)

    FC experiment of human PBMCs using FITC-65202

    1X10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (FITC-65202, Clone: KP1) (right), or Mouse IgG1 Isotype Control (left). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Monocytes were gated.

Tested Applications

Positive FC (Intra) detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65202 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Lysosomal fraction of human alveolar macrophages
Full Name CD68 molecule
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD68 antibody FITC-65202Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC (Intra)

Front Bioeng Biotechnol

Evaluation of nanoparticle albumin-bound paclitaxel loaded macrophages for glioblastoma treatment based on a microfluidic chip

Authors - Zuorun Xie
View Article