1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
FC experiment of human PBMCs using CL405-65202
1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.
Product Information
CL405-65202 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.
1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
FC experiment of human PBMCs using CL405-65202
1X10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 405 Anti-Human CD68 (CL405-65202, Clone:KP1) or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.