CoraLite®594 Anti-Human CD68 (KP1)

CD68 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

KP1

Cat no : CL594-65202

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

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Validation Data Gallery

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  FC experiment of human PBMCs using CL594-65202

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human CD68 (CL594-65202, Clone:KP1) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65202

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite®594 Anti-Human CD68 (CL594-65202, Clone:KP1) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124, Clone: MOPC-21). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011) prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65202 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.