FITC Anti-Human CD206 (15-2)

CD206 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

FITC Fluorescent Dye

Publications

6

CloneNo.

15-2

Cat no : FITC-65155

Synonyms

hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D

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Validation Data Gallery

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  FC experiment of human monocyte-derived dendritic cells using FITC-65155

  Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 4 ul FITC Anti-Human CD206 (FITC-65155, Clone: 15-2) (green) or FITC-Mouse IgG1 isotype control (black). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human monocyte-derived dendritic cells cells

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 6 publications below

Product Information

FITC-65155 targets CD206 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified human mannose receptor
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: CD206
• Gene ID (NCBI): 4360
• RRID: AB_2920499
• Conjugate: FITC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 494 nm / 520 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publications

Species	Application	Title
human	FC	Biochim Biophys Acta Mol Basis Dis; Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages; Authors - Ling Zhou
	FC	Front Immunol; Chamaejasmenin B, an Inhibitor for Metastatic Outgrowth, Reversed M2-Dominant Macrophage Polarization in Breast Tumor Microenvironment.; Authors - Qi Li
	FC	Pharm Biol; Schizandrin A ameliorates cognitive functions via modulating microglial polarisation in Alzheimer's disease mice.; Authors - Qi Wang
	FC	Cancer Sci; Homeobox A7 promotes esophageal squamous cell carcinoma progression through C-C motif chemokine ligand 2-mediated tumor-associated macrophage recruitment; Authors - Anqi Feng
human	FC	J Biochem Mol Toxicol; KLF4-induced upregulation of SOCS1 ameliorates myocardial ischemia/reperfusion injury by attenuating AC16 cardiomyocyte damage and enhancing M2 macrophage polarization; Authors - Jiming Zhou
	FC	Authors -