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FITC Plus Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

MOPC-21

Cat no : FITC-65124

Synonyms

FITC Mouse IgG1 Isotype Control, IgG1, IgG1 Isotype Control, mouse IgG1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Mouse IgG1 Isotype Control (MOPC-21) (FITC-65124)

    FC experiment of human PBMCs using FITC-65124

    1X10^6 human PBMCs were surface stained with 0.2 ug FITC Anti-Mouse IgG1 Isotype Control (FITC-65124, Clone:MOPC-21) (red), FITC Anti-Human CD45RO (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

FITC-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Gene Symbol IgG1 Isotype Control
Gene ID (NCBI) 16017
RRIDAB_2883790
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for FITC Plus IgG1 Isotype Control antibody FITC-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
FC

Int Immunopharmacol

Neutrophil PPIF exacerbates lung ischemia-reperfusion injury after lung transplantation by promoting calcium overload-induced neutrophil extracellular traps formation

Authors - Wensi Wu
View Article