APC Anti-Human CD206 (15-2)

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  FC experiment of human PBMCs using APC-65155

  1x10^6 GM-CSF treated human PBMCs were surface stained with 5 ul APC Anti-Human CD206 (15-2) (APC-65155, Clone:15-2) (red) or APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD206 (15-2) (APC-65155, Clone:15-2) (black, dashed). Cells were not fixed.

CD206 Monoclonal Antibody for FC

Cat No. APC-65155

Clone No.15-2

Publications(3)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Synonyms

hMR, C-type lectin domain family 13 member D-like, C-type lectin domain family 13 member D, CLEC13DL, CLEC13D

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Tested Applications

• Positive FC detected in: GM-CSF treated human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

APC-65155 targets CD206 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Purified human mannose receptor
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 166 kDa
• GenBank Accession Number: NM_002438
• Gene Symbol: CD206
• Gene ID (NCBI): 4360
• RRID: AB_2918930
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P22897
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.

Publications

Species	Application	Title
	FC	Int J Mol Sci; C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis; Authors - Xinyi Qu
human	FC	Cell Mol Life Sci; Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma; Authors - Quan Yan
human	FC	Front Immunol; Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities; Authors - Chaochao Dai