Bcl-XL Monoclonal antibody, PBS Only

Bcl-XL Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4C12A6

Cat no : 66020-1-PBS

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

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Validation Data Gallery

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  WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

  Jurkat, Ramos, K-562 and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66020-1-Ig (same clone as 66020-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66020-1-Ig (same clone as 66020-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66020-1-Ig (Bcl-xL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66020-1-Ig (Bcl-XL antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 66020-1-Ig(Bcl-xL antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  Immunofluorescent analysis of Hela cells, using BCL2L1 antibody 66020-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66020-1-Ig (same clone as 66020-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Bcl-XL (66020-1-Ig, Clone:4C12A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66020-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66020-1-PBS targets Bcl-XL in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Bcl-XL fusion protein Ag18037
• Full Name: BCL2-like 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC019307
• Gene Symbol: BCL2L1
• Gene ID (NCBI): 598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.