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CoraLite®555-conjugated Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4C12A6

Cat no : CL555-66020

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated Bcl-XL Monoclonal antibody (CL555-66020)

    IF Staining of HeLa using CL555-66020

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 Bcl-XL antibody (CL555-66020, Clone: 4C12A6 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite®555-conjugated Bcl-XL Monoclonal antibody (CL555-66020)

    FC experiment of Jurkat using CL555-66020

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human Bcl-XL (CL555-66020, Clone:4C12A6) (red), or 0.4 ug CoraLite® Plus 555 Mouse IgG2b Isotype Control (MPC-11) (CL555-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66020 targets Bcl-XL in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Bcl-XL fusion protein Ag18037
Full Name BCL2-like 1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
RRIDAB_2919668
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

Protocols

Product Specific Protocols
IF protocol for CL555 Bcl-XL antibody CL555-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols