• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite®568-conjugated B23/NPM1 Monoclonal antibody

B23/NPM1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®568 Fluorescent Dye

CloneNo.

4F12A3

Cat no : CL568-60096

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®568-conjugated B23/NPM1 Monoclonal antibo (CL568-60096)

    IF Staining of HepG2 using CL568-60096

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®568 B23/NPM1 antibody (CL568-60096, Clone: 4F12A3 ) at dilution of 1:1000.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®568-conjugated B23/NPM1 Monoclonal antibo (CL568-60096)

    FC experiment of HeLa using CL568-60096

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®568 Anti-Human B23/NPM1 (CL568-60096, Clone:4F12A3) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL568-60096 targets B23/NPM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen B23/NPM1 fusion protein Ag7415
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_2919713
Conjugate CoraLite®568 Fluorescent Dye
Excitation/Emission Maxima Wavelengths568 nm / 587 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Protocols

Product Specific Protocols
IF protocol for CL568 B23/NPM1 antibody CL568-60096Download protocol
FC protocol for CL568 B23/NPM1 antibody CL568-60096Download protocol
Standard Protocols
Click here to view our Standard Protocols