CoraLite®532-conjugated B23/NPM1 Monoclonal antibody

• Featured Product
• KD/KO Validated

B23/NPM1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®532 Fluorescent Dye

CloneNo.

4F12A3

Cat no : CL532-60096

Synonyms

B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Validation Data Gallery

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  IF Staining of HeLa using CL532-60096

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®532 B23/NPM1 antibody (CL532-60096, Clone: 4F12A3 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL532-60096 targets B23/NPM1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: B23/NPM1 fusion protein Ag7415
• Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC002398
• Gene Symbol: NPM1
• Gene ID (NCBI): 4869
• RRID: AB_2919580
• Conjugate: CoraLite®532 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 537 nm / 560 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.