CoraLite®555-conjugated B23/NPM1 Monoclonal antibody

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• KD/KO Validated

B23/NPM1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Publications

1

CloneNo.

4F12A3

Cat no : CL555-60096

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

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Validation Data Gallery

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  IF Staining of HepG2 using CL555-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 B23/NPM1 antibody (CL555-60096, Clone: 4F12A3 ) at dilution of 1:1000. DAPI (blue).

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  FC experiment of HeLa using CL555-60096

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human B23/NPM1 (CL555-60096, Clone:4F12A3) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL555-60096 targets B23/NPM1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: B23/NPM1 fusion protein Ag7415
• Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC002398
• Gene Symbol: NPM1
• Gene ID (NCBI): 4869
• RRID: AB_2919656
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publications

Species	Application	Title
human	IF	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi

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