CoraLite®594-conjugated B23/NPM1 Monoclonal antibody

• Featured Product
• KD/KO Validated

B23/NPM1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

4F12A3

Cat no : CL594-60096

Synonyms

B23, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using the CoraLite®594-conjugated version of this antibody, CL594-60096 (B23/NPM1 antibody), at dilution of 1:100. F-actin is stained using CL488-phalloidin (Green) and DNA is stained by DAPI (blue).

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  IF Staining of HepG2 using CL594-60096

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-60096 (B23/NPM1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-60096 targets B23/NPM1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: B23/NPM1 fusion protein Ag7415
• Full Name: nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: BC002398
• Gene Symbol: NPM1
• Gene ID (NCBI): 4869
• RRID: AB_2883422
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

Publications

Species	Application	Title
	IF	J Hematol Oncol; METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation; Authors - Meilin Xue