HRP-conjugated beta Actin Antibody 1 Publications

Mouse Monoclonal| Catalog number: HRP-60008

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Con: 50 μg/100 μl

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Species specificity:
human, mouse, rat, zebrafish

Positive WB detected in:
HeLa cells

Recommended dilution:
WB : 1:2000-1:20000

Product Information


Source:
Mouse

Purification method:
Caprylic acid/ammonium sulfate precipitation

Isotype:
IgM

Storage:
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC. 20uL sizes contain 0.05% BSA. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Recombinant Protein

Full name:
actin, beta

Calculated molecular weight:
375aa,42 kDa

Observed molecular weight:
42 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
ACTB

Synonyms
ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin
Background

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


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