Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

Unconjugated

CloneNo.

MOPC-21

Cat no : 65124-1-Ig

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 65124-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Mouse IgG1 Isotype Control (65124-1-Ig, Clone:MOPC-21) (red) or 0.2ug Anti-Human CD8a (65113-1-Ig, Clone:Hit8a) (green) and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-8) at dilution 1:1000 or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65124-1-Ig targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: IgG1 Isotype Control
• Gene ID (NCBI): 16017
• RRID: AB_2918418
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.