PerCP-Cyanine5.5-conjugated IgG1 Isotype Control Monoclonal antibody

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

n/a

Applications

FC

Conjugate

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

MOPC-21

Cat no : CPY5-65124

Synonyms

Ighg1, MOPC-21, Igh-4, IgG1

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Validation Data Gallery

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  FC experiment of human PBMCs using CPY5-65124

  1x10^6 human PBMCs were surface stained with 0.1 ug PerCP-Cyanine5.5 Mouse IgG1 Isotype Control (MOPC-21) (CPY5-65124, Clone: MOPC-21) (red), or PerCP-Cyanine5.5 Anti-Human CD45 (HI30) (CPY5-65109, Clone: HI30) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CPY5-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with n/a samples.

• Tested Reactivity: n/a
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: IgG1 Isotype Control
• Gene ID (NCBI): 16017
• Conjugate: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 482 nm / 690nm
• Form: Liquid
• Purification Method: Unknow
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.