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FITC Plus Anti-Human CD3 (UCHT1)

CD3 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

UCHT1

Cat no : FITC-65151

Synonyms

CD3, CD3E, T3E, TCRE

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD3 (UCHT1) (FITC-65151)

    FC experiment of human PBMCs using FITC-65151

    1X10^6 human PBMCs were surface co-stained with CL647 Plus Anti-Human CD19 and 5 ul FITC Plus Anti-Human CD3 (FITC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated

  • Flow cytometry (FC) experiment of human PBMCs using FITC Plus Anti-Human CD3 (UCHT1) (FITC-65151)

    FC experiment of human PBMCs using FITC-65151

    1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD3 (FITC-65151, Clone:UCHT1) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using FITC Plus Anti-Human CD3 (UCHT1) (FITC-65151)

    FC experiment of human peripheral blood lymphocytes using FITC-65151

    1X10^6 human peripheral blood lymphocytes were surface stained with 1 ug FITC-anti-human CD3 (FITC-65151, clone UCHT1) (red) or 1 ug FITC-mouse IgG1 isotype control (blue). Cells were not fixed.

Tested Applications

Positive FC detected inhuman PBMCs, human peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65151 targets CD3 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3E
Gene ID (NCBI) 916
RRIDAB_2883808
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for FITC Plus CD3 antibody FITC-65151Download protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Macarena Lucia (Verified Customer) (09-28-2022)

Good staining at recommended dilution

  • Applications: Flow Cytometry
  • Cell Tissue Type: primary T cell