CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

MOPC-21

Cat no : CL488-65124

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

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Validation Data Gallery

• 

  FC experiment of human PBMCs using CL488-65124

  1X10^6 human PBMCs were surface stained with 0.2 ug CoraLite® Plus 488 Anti-Mouse IgG1 Isotype Control (CL488-65124, Clone: MOPC-21) (red), CL488 Anti-Human CD3 (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

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Tested Applications

Positive FC detected in

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: immunoglobulin heavy constant gamma 1 (G1m marker)
• Gene Symbol: IgG1 Isotype Control
• Gene ID (NCBI): 16017
• RRID: AB_2919265
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.