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CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A3

Cat no : CL488-65209

Synonyms

IgG2a, IgG2a Isotype Control, Rat IgG2a Isotype Control

Validation Data Gallery

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  • Flow cytometry (FC) experiment of C57BL/6 mouse splenocytes using CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209)

    FC experiment of C57BL/6 mouse splenocytes using CL488-65209

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Rat IgG2a Isotype Control (CL488-65209, Clone:2A3) (red), or 0.5 ug CoraLite® Plus 488 Anti-Mouse CD16/32 (93) (CL488-65057, Clone: 93) (green) or unstained (blue). Cells were not fixed.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65209 targets IgG2a Isotype Control in FC applications and shows reactivity with samples.

Tested Reactivity
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name IgG2a Isotype Control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2919272
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IgG2a Isotype Control antibody CL488-65209Download protocol
Standard Protocols
Click here to view our Standard Protocols