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CoraLite® Plus 488 Anti-Mouse CD16/32 (93)

CD16/32 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2a, lambda

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

93

Cat no : CL488-65057

Synonyms

AI528646, CD16/32, CD32, F630109E10Rik, Fc[g]RII, Fcgr2, Fcgr2a, Fcgr2b, FcgRII, Fcr 2, Fcr 3, Ly 17, Ly m20, LyM 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD16/32 (93) (CL488-65057)

    IF Staining of mouse splenocytes using CL488-65057

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD16/32 antibody (CL488-65057, Clone: 93 ) at dilution of 1:100.

  • Flow cytometry (FC) experiment of BALB/c mouse splenocytes using CoraLite® Plus 488 Anti-Mouse CD16/32 (93) (CL488-65057)

    FC experiment of BALB/c mouse splenocytes using CL488-65057

    1X10^6 BALB/c mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD16/32 (CL488-65057, Clone:93) or 0.5 ug Isotype Control. Cells were not fixed.

Tested Applications

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65057 targets CD16/32 in IF/ICC, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2a, lambda
Class Monoclonal
Type Antibody
Immunogen VH81X Tg B cells and T220 stromal cell line
Full Name Fc receptor, IgG, low affinity IIb
GenBank Accession NumberBC038070
Gene Symbol CD16/32
Gene ID (NCBI) 14130
RRIDAB_2919253
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD16/32 antibody CL488-65057Download protocol
Standard Protocols
Click here to view our Standard Protocols