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Anti-Human CD9 Rabbit Recombinant Antibody

CD9 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240830E6

Cat no : 98095-1-RR

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR)

    FC experiment of human PBMCs using 98095-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Human CD3 (OKT3) . Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR)

    FC experiment of human PBMCs using 98095-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR)

    FC experiment of human PBMCs using 98095-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98095-1-RR

    Affinity and Kinetic Characterization of 98095-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98095-1-RR against Human CD9 were performed. The affinity constant is 407 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98095-1-RR is CD9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD9 molecule
Calculated Molecular Weight25 kDa
GenBank Accession NumberNM_001769.4
Gene Symbol CD9
Gene ID (NCBI) 928
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).

Protocols

Product Specific Protocols
FC protocol for CD9 antibody 98095-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols