Anti-Human CD9 Rabbit Recombinant Antibody, PBS Only

CD9 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240830E6

Cat no : 98095-1-PBS

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using 98095-1-RR (same clone as 98095-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Human CD3 (OKT3) . Cells were not fixed. This data was developed using the same antibody clone with 98095-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98095-1-RR (same clone as 98095-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6)(red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98095-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98095-1-RR (same clone as 98095-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD9 Rabbit Recombinant Antibody (98095-1-RR, Clone:240830E6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98095-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98095-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98095-1-RR against Human CD9 were performed. The affinity constant is 0.184 nM.

---

Product Information

The immunogen of 98095-1-PBS is CD9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD9 molecule
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_001769.4
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).