CoraLite® Plus 750 Anti-Human CD9 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL750-98095

  1x10^6 human PBMCs were surface stained with PE Anti-Human CD3 and 5 ul CoraLite® Plus 750 Anti-Human CD9 Rabbit RecAb (CL750-98095, Clone:240830E6), or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98095

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD9 Rabbit RecAb (CL750-98095, Clone:240830E6)(red), or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9)(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD9 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-98095

Clone No.240830E6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD9 antigen, BTCC 1, BA2, 5H9 antigen, 5H9

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98095 targets CD9 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD9 molecule
• Calculated Molecular Weight: 25 kDa
• GenBank Accession Number: NM_001769.4
• Gene Symbol: CD9
• Gene ID (NCBI): 928
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21926
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). The CD9 antigen appears to be a 227-amino acid molecule with four hydrophobic domains and one N-glycosylation site (PMID: 1840589). This antibody detects bands of 23-30 kDa, it may be due to the difference of glycosylations (PMID: 8701996).