• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-WWTR1

WWTR1 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

N° de cat : 23306-1-AP

Synonymes

TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif



Applications testées

Résultats positifs en WBcellules HeLa, cellules LN229, cellules MDA-MB-453s
Résultats positifs en IPcellules MDA-MB-453s,
Résultats positifs en IF-Ptissu rénal de rat,
Résultats positifs en IF/ICCcellules U-251,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

23306-1-AP cible WWTR1 dans les applications de WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat

Réactivité Humain, rat
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène WWTR1 Protéine recombinante Ag19659
Nom complet WW domain containing transcription regulator 1
Masse moléculaire calculée 44 kDa
Poids moléculaire observé 50 kDa
Numéro d’acquisition GenBankBC014052
Symbole du gène WWTR1
Identification du gène (NCBI) 25937
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protocole

Product Specific Protocols
WB protocol for WWTR1 antibody 23306-1-APDownload protocol
IF protocol for WWTR1 antibody 23306-1-APDownload protocol
IP protocol for WWTR1 antibody 23306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF,IP

Cancer Cell

Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis.

Authors - Lai Man Natalie Wu
humanWB,IHC

Nat Neurosci

YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells.

Authors - Yannick Poitelon
  • KO Validated
humanWB

Adv Sci (Weinh)

DNMT3A Cooperates with YAP/TAZ to Drive Gallbladder Cancer Metastasis

Authors - Sunwang Xu
mouse,humanWB

Sci Adv

YAP drives fate conversion and chemoresistance of small cell lung cancer.

Authors - Qingzhe Wu
  • KD Validated
mouseWB,IF

Bone Res

TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.

Authors - Wanlei Yang
  • KO Validated
humanWB

Clin Transl Med

NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization

Authors - Shaomin Zou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yohanes (Verified Customer) (04-24-2023)

  • Applications: Western Blot, Immunofluorescence, Other
  • Primary Antibody Dilution: 1:1000 for WB, 1:200 for IF, 1:100 for PLA
  • Cell Tissue Type: bEnd.3 mouse Endothelial cell
FH

Joshua (Verified Customer) (02-28-2020)

MDCKs fixed in 4% paraformaldehyde. Bright Mix of nuclear and cytosolic staining

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: MDCK
WWTR1 Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:23306-1-AP)