PAX3 Polyclonal antibody

PAX3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21386-1-AP

Synonyms

CDHS, HUP2, paired box 3, Paired box protein Pax 3, PAX3, WS1

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Validation Data Gallery

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  WB analysis of mouse skin using 21386-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21386-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21386-1-AP (PAX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 21386-1-AP

  IP result of anti-PAX3 (IP:21386-1-AP, 4ug; Detection:21386-1-AP 1:500) with mouse skin tissue lysate 3200ug.

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Tested Applications

• Positive WB detected in: mouse skin tissue, HEK-293 cells
• Positive IP detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 21386-1-AP is PAX3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAX3 fusion protein Ag16021
• Full Name: paired box 3
• Calculated Molecular Weight: 484 aa, 53 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: BC101301
• Gene Symbol: PAX3
• Gene ID (NCBI): 5077
• RRID: AB_10732817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.

Publications

Species	Application	Title
hamster	IHC	Birth Defects Res; Cigarette smoke induced neural tube defects by down-regulating noggin expression.; Authors - Chuanhui Yin
mouse	WB	Neurochem Int; Cycloleucine induces neural tube defects by reducing Pax3 expression and impairing the balance of proliferation and apoptosis in early neurulation; Authors - Li Zhang