ZO-2 Polyclonal antibody

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  WB analysis of HeLa using 18900-1-AP

  WB result of ZO-2 antibody (18900-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZO-2 transfected HeLa cells.

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  WB analysis using 18900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18900-1-AP (ZO-2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18900-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18900-1-AP (ZO-2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18900-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18900-1-AP (ZO-2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18900-1-AP (ZO-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18900-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18900-1-AP (ZO-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 18900-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18900-1-AP (ZO-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 18900-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-2 antibody (18900-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 18900-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ZO-2 antibody (18900-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 18900-1-AP

  Immunofluorescent analysis of MCF-7 cells, using TJP2 antibody 18900-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

ZO-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18900-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TJP2, ZO2, ZO 2, TJP 2, Tight junction protein ZO-2

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse brain tissue, rat brain tissue, NIH/3T3 cells, C6 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

18900-1-AP targets ZO-2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ZO-2 fusion protein Ag13534
• Full Name: tight junction protein 2 (zona occludens 2)
• Calculated Molecular Weight: 134 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC027592
• Gene Symbol: TJP2
• Gene ID (NCBI): 9414
• RRID: AB_2203584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UDY2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei.

What is the molecular weight of ZO-2? Are there any isoforms of ZO-2? Is ZO-2 post-translationally modified?

The molecular weight of ZO-2 is 160 kDa. There are two known isoforms of ZO-2: full-length ZO-2A and ZO-2C lacking the first 23 amino acids (PMID: 10360833). ZO-2 can be phosphorylated and O-glycosylated, which has an impact on its localization (PMID: 23804652).

What is the subcellular localization of ZO-2?

ZO-2 is present at the cytoplasmic face at cell-cell contact sites: tight junctions, adherens junctions, and gap junctions (PMID: 20224657). ZO-2 contains nuclear localization and nuclear export signals and can actively shuttle between the cytoplasm and nucleus (PMID: 11855865). In the nucleus it can affect the activity of various transcription factors. ZO-2 nuclear localization is most prominent in sparse 2D cell cultures and also in cells subjected to mechanical or chemical stress.

What is the tissue expression pattern of ZO-2?

ZO-2 is expressed in various tissues in epithelial and endothelial cells. ZO-2 isoforms have tissue specificity, with ZO-2A being abundant in the heart and brain, while ZO-2C is found in the kidney, pancreas, heart, and placenta (PMID: 11018256).

What proteins can be used as markers of tight junctions?

Zona occludens proteins are often used as tight junction markers. Apart from the antibody against ZO-2, one can use antibodies against ZO-1 (https://www.ptglab.com/products/ZO1-Antibody-21773-1-AP.htm), occludin (https://www.ptglab.com/products/OCLN-Antibody-13409-1-AP.htm), and claudins (e.g., claudin 1: https://www.ptglab.com/products/CLDN1-Antibody-13050-1-AP.htm).

Publications

Species	Application	Title
mouse	IF	EMBO Mol Med; Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.; Authors - Ming Zhao
human	WB	Cell Rep; p120 RasGAP and ZO-2 are essential for Hippo signaling and tumor-suppressor function mediated by p190A RhoGAP; Authors - Hanyue Ouyang ; KD Validated
mouse	IF	Biomater Sci; Improved epithelial cell-cell adhesion using molecular mobility of supramolecular surfaces.; Authors - Ryo Mikami
human	WB	Free Radic Biol Med; Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.; Authors - Restituto Tocmo
mouse	WB,IHC	J Agric Food Chem; Gut Microbiota from Short-Chain Chlorinated Paraffin-Exposed Mice Promotes Astrocyte Activation by Disrupting the Intestinal Tight Junction via Zonulin Upregulation; Authors - Xinyuan Zhao
	WB	J Cereb Blood Flow Metab; NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke.; Authors - Fan Yang