PAX8 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAX8 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

516

Reviews (6)

Cat no : 10336-1-AP

Synonyms

PAX-8, PAX 8, Paired box protein Pax-8, Paired box protein Pax 8, paired box 8

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Validation Data Gallery

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  WB analysis of SKOV-3 using 10336-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10336-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10336-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10336-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10336-1-AP (PAX8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 10336-1-AP

  IP result of anti-PAX8 (IP:10336-1-AP, 4ug; Detection:10336-1-AP 1:15000) with SKOV-3 cells lysate 3280 ug.

• 

  IP experiment of HeLa cells using 10336-1-AP

  IP result of anti-PAX8 (10336-1-AP for IP and Detection) with Hela cell.

• 

  IHC staining of human thyroid cancer using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 10336-1-AP (PAX8 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10336-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10336-1-AP (PAX8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 10336-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using PAX8 antibody (10336-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 10336-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10336-1-AP (PAX8 antibody) at dilution of 1:600 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SKOV-3 using 10336-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PAX8 antibody (10336-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: SKOV-3 cells, HEK-293 cells, Raji cells, NIH/3T3 cells
• Positive IP detected in: SKOV-3 cells, HeLa cells
• Positive IHC detected in: human ovary cancer tissue, human renal cell carcinoma tissue, human thyroid cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 55 publications below
• IHC: See 375 publications below
• IF: See 109 publications below
• ELISA: See 1 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Product Information

10336-1-AP targets PAX8 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, rabbit, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: paired box 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa, 58 kDa
• GenBank Accession Number: BC001060
• Gene Symbol: PAX8
• Gene ID (NCBI): 7849
• RRID: AB_2236705
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

This antibody can detect all 5 isoforms of PAX8 (31 kDa, 34 kDa, 41 kDa, 43 kDa and 48 kDa). A 58-60 kDa form has also been reported (PMID:15650356, 1049952). Proteintech's 10336-1-AP antibody has cross-reactivities with the other PAX family members.

Publications

Species	Application	Title
human	WB,CoIP	Nature; The renal lineage factor PAX8 controls oncogenic signalling in kidney cancer.; Authors - Saroor A Patel ; KD Validated
human	IHC	Nat Biotechnol; Tubuloids derived from human adult kidney and urine for personalized disease modeling.; Authors - Frans Schutgens
human	IHC	Nat Med; Combined mutation in Vhl, Trp53 and Rb1 causes clear cell renal cell carcinoma in mice.; Authors - Sabine Harlander
human,mouse	WB,IHC	Cancer Discov; A Targetable Myeloid Inflammatory State Governs Disease Recurrence in Clear Cell Renal Cell Carcinoma.; Authors - Phillip M Rappold
human	IHC	Cell Stem Cell; Patient-derived organoids model cervical tissue dynamics and viral oncogenesis in cervical cancer.; Authors - Kadi Lõhmussaar
human	IF	Cell Stem Cell; Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network.; Authors - Jian Hui Low

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

Worked well for immunolabeling on mouse retina tissue sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Retina

FH

Eunyoung (Verified Customer) (02-28-2020)

This antibody works well on the mouse ovarian cancer tissue

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovary /fallopian tissue

PAX8 Antibody Immunohistochemistry, validation (1:1000 dilution) in Ovary /fallopian tissue (Cat no:10336-1-AP)

FH

shuang (Verified Customer) (02-18-2020)

This Pax8 antibody works great for both IHC and IF staining on mouse Fallopian tube/uterus organoids and tumors, Highly recommend!

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse Fallopian tube/ Fallopian tube organoids

FH

Adam (Verified Customer) (05-30-2019)

Antibody works great for our applications (IHC on FFPE tissues). Only comment is that it cane sometimes have some background staining, particularly in some mouse tissues such as liver.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:1000

FH

Huai-Chin (Verified Customer) (04-15-2019)

So far only try the antibody for IHC on mouse tissue. Works pretty good. Would like to try it on human tissue/cell lines.

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse Ovary/Oviduct

FH

Paulo (Verified Customer) (03-03-2019)

Used to perform ChIP-seq in ccRCC cell lines. 17.5ug of antibody used in 100million cells. IP was validated by WB. Sequencing detected PAX8 binding genome wide. Motif analysis identified the consensus PAX8 binding motif in approximately 90% of detected peaks.Would be great if Proteintech would produce a more concentrated version of this antibody for ChIP-seq.

• Applications: ChIP,

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