Anticorps Polyclonal de lapin anti-VAMP3/Cellubrevin

• Phare
• Validé par KD/KO

VAMP3/Cellubrevin Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA, Blocking

Conjugaison

Non conjugué

Publications(20)

N° de cat : 10702-1-AP

Synonymes

VAMP3, CEB, Cellubrevin, SYB3, Synaptobrevin-3

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Galerie de données de validation

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  WB analysis of HEK-293 using 10702-1-AP

  WB result of VAMP3/Cellubrevin antibody (10702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAMP3/Cellubrevin transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 10702-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10702-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10702-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10702-1-AP

  IP result of anti-VAMP3/Cellubrevin (IP:10702-1-AP, 4ug; Detection:10702-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of mouse testis using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10702-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 8 publications below

Informations sur le produit

10702-1-AP cible VAMP3/Cellubrevin dans les applications de WB, IHC, IF, IP, ELISA, Blocking et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VAMP3/Cellubrevin Protéine recombinante Ag1156
• Nom complet: vesicle-associated membrane protein 3 (cellubrevin)
• Masse moléculaire calculée: 11 kDa
• Poids moléculaire observé: 11-17 kDa
• Numéro d’acquisition GenBank: BC005941
• Symbole du gène: VAMP3
• Identification du gène (NCBI): 9341
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

Publications

Species	Application	Title
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
rat	WB,IF	Aging Cell; Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.; Authors - Zunshu Du
human	WB,IF	Oncogene; Syntaxin-6 mediated autophagy confers lenvatinib resistance in hepatocellular carcinoma; Authors - Guo-Pei Zhang
human	IHC	Oncotarget; A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis.; Authors - Mitsuhiro Shimura

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