VAMP1 Polyclonal antibody

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  WB analysis of human brain using 13115-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13115-1-AP (VAMP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 13115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13115-1-AP (VAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 13115-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 13115-1-AP (VAMP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

VAMP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 13115-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Vesicle-associated membrane protein 1, VAMP-1, VAMP 1, Synaptobrevin-1, Synaptobrevin 1

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

13115-1-AP targets VAMP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAMP1 fusion protein Ag3787
• Full Name: vesicle-associated membrane protein 1 (synaptobrevin 1)
• Calculated Molecular Weight: 117 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC023286
• Gene Symbol: VAMP1
• Gene ID (NCBI): 6843
• RRID: AB_2214772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23763
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP1, also named as synaptobrevin 1, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP1 is a part of the SNARE (soluble NSF-attachment protein receptor) complex. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP1 is involved in synaptic vesicle exocytosis, a fundamental step in neurotransmitter release.

Publications

Species	Application	Title
mouse	WB	Pharmacol Res; The role of circTmeff-1 in incubation of context-induced morphine craving.; Authors - Hailei Yu
mouse	IF	Eur J Immunol; ACKR1 favors transcellular over paracellular T-cell diapedesis across the blood-brain barrier in neuroinflammation in vitro.; Authors - Luca Marchetti
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
mouse	IF	EMBO J; Binary organization of epidermal basal domains highlights robustness to environmental exposure; Authors - Sangeeta Ghuwalewala
mouse	WB	Neurotoxicology; Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice; Authors - Danyang Li