SNAP23 Polyclonal antibody

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  WB analysis of HeLa using 10825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10825-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10825-1-AP

  IP result of anti-SNAP23 (IP:10825-1-AP, 4ug; Detection:10825-1-AP 1:500) with HeLa cells lysate 880ug.

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  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10825-1-AP (SNAP23 antibody) at dilution of 1:50 (under 10x lens).

• Featured Product
• KD/KO Validated

SNAP23 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10825-1-AP

Publications(22)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Synaptosomal-associated protein 23, SNAP23B, SNAP23A, SNAP-23, SNAP 23

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Product Information

10825-1-AP targets SNAP23 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAP23 fusion protein Ag1269
• Full Name: synaptosomal-associated protein, 23kDa
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC000148
• Gene Symbol: SNAP23
• Gene ID (NCBI): 8773
• RRID: AB_2192022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00161
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
rat	IF	Aging Cell; Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.; Authors - Zunshu Du
mouse	IF	Cell Death Differ; Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.; Authors - Zhaokang Cui
human	WB	Oncogene; Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.; Authors - Chengdong Liu ; KD Validated
human	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shweta (Verified Customer) (11-18-2024)

Antibody recognizes the correct band size and works great in WB

• Applications: Western Blot