SNAP23 Polyclonal antibody

• Featured Product
• KD/KO Validated

SNAP23 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 10825-1-AP

Synonyms

HsT17016, SNAP 23, SNAP23, SNAP23A, SNAP23B

---

Validation Data Gallery

• 

  WB analysis of HeLa using 10825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10825-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10825-1-AP (SNAP23 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10825-1-AP

  IP Result of anti-SNAP23 (IP:10825-1-AP, 4ug; Detection:10825-1-AP 1:500) with HeLa cells lysate 880ug.

• 

  IHC staining of human prostate cancer using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10825-1-AP (SNAP23 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 10825-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10825-1-AP (SNAP23 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10825-1-AP

  Immunofluorescent analysis of HepG2 cells, using SNAP23 antibody 10825-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 10825-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SNAP23 (10825-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Product Information

10825-1-AP targets SNAP23 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNAP23 fusion protein Ag1269
• Full Name: synaptosomal-associated protein, 23kDa
• Calculated molecular weight: 23 kDa
• Observed molecular weight: 23-25 kDa
• GenBank accession number: BC000148
• Gene symbol: SNAP23
• Gene ID (NCBI): 8773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu ; KO Validated
human	WB,IF	Nat Commun; KRAS regulation by small non-coding RNAs and SNARE proteins.; Authors - Yonglu Che
rat	IF	Aging Cell; Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor.; Authors - Zunshu Du
mouse	IF	Cell Death Differ; Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.; Authors - Zhaokang Cui
human	WB	Oncogene; Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene.; Authors - Chengdong Liu ; KD Validated
human	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu

Related Products

Syntaxin 4 Polyclonal antibody

14988-1-AP

VAMP2 Polyclonal antibody

10135-1-AP

SNAP25 Polyclonal antibody

14903-1-AP

SNAP25 Monoclonal antibody

60159-1-Ig

VAMP3/Cellubrevin Polyclonal antibody

10702-1-AP