VAMP2 Polyclonal antibody

VAMP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA, Blocking

Conjugate

Unconjugated

Publications

26

Cat no : 10135-1-AP

Synonyms

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Validation Data Gallery

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  WB analysis using 10135-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 10135-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10135-1-AP (VAMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10135-1-AP (VAMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10135-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 10135-1-AP (VAMP2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 12 publications below

Product Information

10135-1-AP targets VAMP2 in WB, IHC, IF, ELISA, Blocking applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VAMP2 fusion protein Ag0179
• Full Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 19 kDa
• GenBank Accession Number: BC002737
• Gene Symbol: VAMP2
• Gene ID (NCBI): 6844
• RRID: AB_2256918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2, with a molecular mass of 15-19 kDa, consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
human	WB,IF	Adv Sci (Weinh); Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage; Authors - Nikita Raj
	IF	Neuron; Rapid Cue-Specific Remodeling of the Nascent Axonal Proteome.; Authors - Roberta Cagnetta
mouse	IHC,IF	Brain; Tau accelerates α-synuclein aggregation and spreading in Parkinson's disease.; Authors - Lina Pan
human	IF	Sci Signal; A hepatokine derived from the ER protein CREBH promotes triglyceride metabolism by stimulating lipoprotein lipase activity; Authors - Hyunbae Kim